Title: Mutations
Course: Biology (B)

Mutations

Objectives and Vocabulary

Lesson Objectives

- Identify causes of mutation.
- Compare and contrast types of mutations.
- Explain how mutations may affect the organisms in which they occur.

Vocabulary

CK-12 Foundation, Biology. http://creativecommons.org/licenses/by-nc-sa/3.0/

Reading Guide

Click here to open Mutation Reading Guide

Introduction

Introduction

A change in the sequence of bases in DNA or RNA is called a mutation. Does the word mutation make you think of science fiction and bug-eyed monsters? Think again. Everyone has mutations. In fact, most people have dozens or even hundreds of mutations in their DNA. Mutations are essential for evolution to occur. They are the ultimate source of all new genetic material in a species. Although most mutations have no effect on the organisms in which they occur, some mutations are beneficial. Even harmful mutations rarely cause drastic changes in organisms.

CK-12 Foundation, Biology. http://creativecommons.org/licenses/by-nc-sa/3.0/

Causes of Mutations

Causes of Mutation

Mutations have many possible causes. Some mutations seem to happen spontaneously without any outside influence. They occur when mistakes are made during DNA replication or transcription. Other mutations are caused by environmental factors. Anything in the environment that can cause a mutation is known as a mutagen. Examples of mutagens are pictured in Figure below.

. http://www.youtube.com/watch?v=0wrNxCGKCws&#38;feature=related
CK-12 Foundation, Biology. http://creativecommons.org/licenses/by-nc-sa/3.0/

Types of Mutations

Mutations

The process of DNA replication is not always 100% accurate, and sometimes the wrong base is inserted in the new strand of DNA. A permanent change in the sequence of DNA is known as a mutation (Figure below).A mutation may have no effect on the phenotype or can cause the protein to be manufactured incorrectly, which can affect how well the protein works, or whether it works at all. Usually the loss of a protein function is detrimental to the organism.

However, in rare circumstances, the mutation can be beneficial. For example, suppose a mutation in an animal’s DNA causes the loss of an enzyme that makes a dark pigment in the animal’s skin. If the population of animals has moved to a light colored environment, the animals with the mutant gene would have a lighter skin color and be better camouflaged. So in this case, the mutation was beneficial.

There are many possible types of mutations possible in chromosomes. In the case of a point mutation, there is a change in a single nucleotide. Other mutations can be more dramatic. A large segment of DNA can be deleted, duplicated, inverted, or inserted in the wrong place. These mutations usually result in a non-functional protein, or a number of non-functional proteins. A deletion is when a segment of DNA is lost, so there is a missing segment in the chromosome. A duplication is when a segment is repeated, creating a longer chromosome. In an inversion, the segment of DNA is flipped and then reattached to the chromosome. An insertion is when a segment of DNA from one chromosome is added to another, unrelated chromosome.

Mutations can arise in DNA through deletion, duplication, inversion, insertion, and translocation within the chromosome. A deletion is when a segment of DNA is lost from the chromosome. A duplication is when a segment is repeated. In an inversion, the segment of DNA is flipped and then re-annealed. An insertion or translocation can cause DNA from one chromosome to be added onto another, unrelated chromosome.

Even if a single base is changed, it can cause a major problem. The substitution of a single base is called a point mutation. Sickle cell anemia is an example of a condition caused by a point mutation in the hemoglobin gene. In this gene, just the one base change causes a different amino acid to be inserted in the hemoglobin protein, causing the protein to fold differently and not function properly in carrying oxygen in the bloodstream.

If a single base is deleted, it can also have huge effects on the organism because this would cause a frameshift mutation. Remember that the bases are read in groups of three by the tRNA. If the reading frame gets off by one base, the resulting sequence will consist of an entirely different set of codons. The reading of an mRNA is like reading three letter words of a sentence. Imagine you wrote “big dog ate red cat”. If you take out the second letter, the frame will be shifted so now it will read “bgd oga ter edc at.” One single deletion makes the whole “sentence”, or mRNA, not read correctly.

Many mutations are not caused by errors in replication. Mutations can happen spontaneously and they can be caused by mutagens in the environment. An example of a mutagen is radiation. High levels of radiation can alter the structure of DNA. Also, some chemicals, such as those found in tobacco smoke, can be mutagens. Sometimes mutagens can also cause cancer. Tobacco smoke, for example, is often linked to lung cancer.

Effects of Mutations

Effects of Mutations

The majority of mutations have neither negative nor positive effects on the organism in which they occur. These mutations are called neutral mutations. Examples include silent point mutations. They are neutral because they do not change the amino acids in the proteins they encode. Many other mutations have no effect on the organism because they are repaired before protein synthesis occurs. Cells have multiple repair mechanisms to fix mutations in DNA. One way DNA can be repaired is illustrated in Figure below. If a cell’s DNA is permanently damaged and cannot be repaired, the cell is likely to be prevented from dividing.

DNA Repair Pathway. This flow chart shows one way that damaged DNA is repaired in E. coli bacteria.

Beneficial Mutations

Some mutations have a positive effect on the organism in which they occur. They are called beneficial mutations. They lead to new versions of proteins that help organisms adapt to changes in their environment. Beneficial mutations are essential for evolution to occur. They increase an organism’s changes of surviving or reproducing, so they are likely to become more common over time. There are several well-known examples of beneficial mutations. Here are just two:

1. Mutations in many bacteria that allow them to survive in the presence of antibiotic drugs. The mutations lead to antibiotic-resistant strains of bacteria.
2. A unique mutation is found in people in a small town in Italy. The mutation protects them from developing atherosclerosis, which is the dangerous buildup of fatty materials in blood vessels. The individual in which the mutation first appeared has even been identified.

Harmful Mutations

Imagine making a random change in a complicated machine such as a car engine. The chance that the random change would improve the functioning of the car is very small. The change is far more likely to result in a car that does not run well or perhaps does not run at all. By the same token, any random change in a gene's DNA is likely to result in a protein that does not function normally or may not function at all. Such mutations are likely to be harmful. Harmful mutations may cause genetic disorders or cancer.

- A genetic disorder is a disease caused by a mutation in one or a few genes. A human example is cystic fibrosis. A mutation in a single gene causes the body to produce thick, sticky mucus that clogs the lungs and blocks ducts in digestive organs.
- Cancer is a disease in which cells grow out of control and form abnormal masses of cells. It is generally caused by mutations in genes that regulate the cell cycle. Because of the mutations, cells with damaged DNA are allowed to divide without limits. Cancer genes can be inherited. You can learn more about hereditary cancer by watching the video at the link below.
- http://www.youtube.com/v/8s4he3wLgkM&#38;feature=PlayList&#38;p=397710758E9BCB24&#38;playnext_from=PL&#38;playnext=1&#38;index=17

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Lesson Summary

Lesson Summary
- Mutations are caused by environmental factors known as mutagens. Types of mutagens include radiation, chemicals, and infectious agents.
- Germline mutations occur in gametes.
- Somatic mutations occur in other body cells.
- Chromosomal alterations are mutations that change chromosome structure.
- Point mutations change a single nucleotide.
- Frameshift mutations are additions or deletions of nucleotides that cause a shift in the reading frame.
- Mutations are essential for evolution to occur because they increase genetic variation and the potential for individuals to differ.
- The majority of mutations are neutral in their effects on the organisms in which they occur.
- Beneficial mutations may become more common through natural selection.
- Harmful mutations may cause genetic disorders or cancer.

CK-12 Foundation, Biology. http://creativecommons.org/licenses/by-nc-sa/3.0/